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Nucleofection® Enables Luciferase Reporter Assays in Primary Cells and Virtually Any Cell Line
Amaxa GMBH
The combination of Promega’s luciferase products and amaxa's Nucleofector® Technology enables researchers to obtain much more significant results with physiologically relevant cells. In this technical note, we give suggestions for Nucleofection® of luciferase constructs and an overview about the wide range of possible applications.
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RNAi-mediated CD2 suppression in primary human T cells
Amaxa GMBH
The down-regulation of gene expression using small interfering RNA (siRNA) has become the method of choice for functional gene analysis in cells. Data presented in this application note show that CD2 expression can be specifically down-regulated in both resting and activated primary human T cells via the Nucleofection® of CD2 specific siRNA molecules.
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First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
Amaxa GMBH
High-throughput transfection of siRNA libraries has become a valuable tool in target identification and validation. However, such screenings have so far been constrained to mostly easy- to-transfect adherent cell lines. amaxa’s Nucleofector® 96-well Shuttle® System extends these approaches to primary and hard-to-transfect cells. In this application note we report results of two different screening approaches in hard-to-transfect cell types using Thermo Scientific Dharmacon siARRAY® siRNA librari
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Transfection of siRNAs into CML primary cells using Nucleofection®
Amaxa GmbH
RNA interference studies are based on transfection of short oligonucleotides (siRNAs) into cells and transfection efficiency is a crucial factor for such experiments. Most conventional transfection methods are suitable for immorta lized cell lines, but are not efficient in primary cells. We performed comparative analysis of transfection methods for siRNA delivery into peripheral blood leukocytes from chronic myeloid leukemia (CML) patients. In conclusion, transfection by amaxa Nucleofector® Tech
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ScreenTape® Enables Fast Quality Control of miRNA qPCR Reactions
AnaSpec
The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required.
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