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NEW TECHNOLOGIES FOR FFPE SAMPLES: Improved RNA Isolation and novel cDNA priming for qPCR and for universal mRNA amplification
G Krupp1; R Jaggi2; D Englert3, DJ Wilson3, S Laken3, S, ES Quabius4. 1AmpTec GmbH, Hamburg, Germany; 2Department of Clinical Research, University of Bern, Switzerland; 3Xceed Molecular, Toronto, Canada; 4Department of Dentistry, UKSH, Kiel, Germany

Improved FFPE RNA isolation, novel TR priming for complete recovery of FFPE mRNA fragments. Differential expression defines Scores for tumor classification: comparing fresh-frozen with FFPE. Novel flow-through Ziplex microarray platform demonstrates MAQC performance level of data obtained with FFPE samples.

FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy
Maryam Mohajeri, Mandana Mohyeddin Bonab, Behrooz Nikbin, Ali Farazmand. Tehran University of Medical Sciences,University of Tehran

Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.

miR-21 upregulation and miR-128a downregulation in human glioblastomas
P. Costa1,2, A. Cardoso1, L. Almeida1,3, M.C. Pedroso de Lima1,2, P. Canoll4, J. Bruce5. 1 Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal 2 Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Apartado 3126, 30

miRNA deregulation is an important hallmark on cancer development and progression. This work shows that miR-21 is overexpressed is human glioblastoma samples and cell lines, whereas miR-128a is highly downregulated on these tumours. miRNA modulation using antisense oligonucleotides and/or miRNA precursor molecules decreased tumor cell viability. Thus, targeting deregulated miRNAs may constitute a complementary/alternative approach on the treatment of glioblastomas and other miRNA-related maligna

Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes with fatty acid metabolism but not with glucose uptake in L6 muscle cells
Constantinescu, S., Turcotte, L.P. University of Southern California

The role of RIP140 in the regulation of fatty acid uptake, oxidation and glucose uptake was investigated in muscle cells using RNAi technique. Cells were incubated ±insulin before measurement of glucose and FA kinetics. We suggest that reducing RIP140 expression in muscle has no impact on the regulation of GU but leads to high rates of FAU and reduced sensitivity to insulin as it pertains to FA metabolism.

Co-rgulation of tumour suppressor gene programmed cell death 4 (PDCD4) by miR-21 and miR-499 in head and neck cancer
Xiaoying Zhang 1, 2, Barbara Rose 1, 2, Rosetta Martiniello-Wilks3 and Nham Tran 1, 2, 3. 1. The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital and University of Sydney, Sydney, NSW 2006, Australia.

This study has identified differentially expressed miRNAs in tonsil cancers and provides new evidence for co-regulation of the oncogene PDCD4 by miR021 and miR-499

MitoProd patented technology for RNA manufacturing and its novel circular interfering RNA
Jérome Lacoste*, Guillaume Plane*, and Jean-Paul Di Rago** . * MitoProd SA, 146 rue Léo Saignat, 33076 Bordeaux, France - ** CNRS (Centre National de la Recherche Scientifique), Bordeaux, France

Here is a description of MitoProd patented technology for RNA manufacturing, permitting a recurring production of RNA in industrial quantities. This technology enables the production of custom RNA at a gram scale, 99 % full length and 95% pure. MitoProd has also designed a new class of interfering RNA called ciRNA®, which have improved features such as RNAses resistance and an increased in vivo efficiency compared to siRNAs.

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